| 余 鸽,龙凤来,张 莹,刘建军.基于R软件包（Polysat）的巴山木竹多倍体植物微卫星数据处理[J].林业调查规划,2023,48(4):33-38 |
| 基于R软件包（Polysat）的巴山木竹多倍体植物微卫星数据处理 |
| Micro-satellite Data Processing of Polyploid Plant Bashania fargesii Based on R Software Package (Polysat) |
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| DOI： |
| 中文关键词: 多倍体 微卫星DNA数据处理 Polysat软件 巴山木竹 |
| 英文关键词: polyploid micro-satellite DNA data processing Polysat software Bashania fargesii |
| 基金项目:杨凌职业技术学院项目（A2019052）；陕西省林业科学院科技创新计划专项项目（SXLK2021-0209）. |
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| 中文摘要: |
| 针对目前多倍体植物的微卫星数据等位基因拷贝数据处理困难、数据格式转换等问题，利用R语言下Polysat软件包，以巴山木竹13个种群260份样本的微卫星数据为例，进行微卫星数据的导入、输出和基因分析，如微卫星等位基因信息统计SSR数据的基因型判断及无性系克隆的划分，种群间的PCA分析，种群间的Fst值分析等。认为与其它多倍体数据处理软件相比，该软件R编程环境具备的多功能性和数据输入、输出方法简单等特性，为相关研究人员提供了方便。 |
| 英文摘要: |
| In response to the difficulties in processing allele copy data and data format conversion of polyploid plants, the Polysat software package in R language was used to import, output, and analyze micro-satellite data from 260 samples of 13 populations of Bashania fargesii, including genotype judgment and clone division of SSR data for micro-satellite allele information statistics, as well as PCA analysis and Fst values analysis between populations. Compared with other polyploid data processing software, the R programming environment of this software had the characteristics of versatility and simplicity of data input and output, providing convenience for relevant researchers. |
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